exosome-mediated delivery of sirna in vitro and in vivo pdf writer

Exosome-mediated Delivery Of Sirna In Vitro And In Vivo Pdf Writer

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Head and neck cancer HNC is one of the most common malignant neoplasms observed worldwide 1. Therefore, it is important to find novel treatment strategies for HNC.

Nanotechnology in targeting cancer stem cells View all 11 Articles. Drug resistance, difficulty in specific targeting and self-renewal properties of cancer stem cells CSCs all contribute to cancer treatment failure and relapse. CSCs have been suggested as both the seeds of the primary cancer, and the roots of chemo- and radio-therapy resistance. The ability to precisely deliver drugs to target CSCs is an urgent need for cancer therapy, with nanotechnology-based drug delivery system being one of the most promising tools to achieve this in the clinic. Exosomes are cell-derived natural nanometric vesicles that are widely distributed in body fluids and involved in multiple disease processes, including tumorigenesis.

Exosomes: Nanoparticulate tools for RNA interference and drug delivery

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Exosomes, membranous nanovesicles, naturally carry bio-macromolecules and play pivotal roles in both physiological intercellular crosstalk and disease pathogenesis. Here, we showed that B cell-derived exosomes can function as vehicles to deliver exogenous miRNA mimic or inhibitor into hepatocytes or macrophages, respectively. Stimulation of B cells significantly increased exosome production.

Exosome-mediated delivery of functionally active miRNA-155 inhibitor to macrophages

Lack of effective tumor-specific delivery systems remains an unmet clinical challenge for successful translation of innovative therapies, such as, therapeutic oligonucleotides. In the past decade, exosomes have been suggested to be ideal drug delivery systems with application in a broad range of pathologies including cancer, due to their organotropic properties. Tumor-derived exosomes, having tumor-homing properties, can efficiently reach cancer cells and therefore behave as carriers for improved drug delivery to the primary tumor and metastases. However, due to their complex composition, and still undefined biological functions, safety concerns arise hampering their translation to the clinics. We propose here the development of exosome-mimetic nanosystems EMNs that simulate natural tumor-derived exosomes with respect to their structure and functionality, but with a controlled composition, for the targeted delivery of therapeutic oligonucleotides to lung adenocarcinoma cells microRNA mimics. EMNs show great similarities to natural exosomes with respect to their physicochemical properties, drug loading capacity, and ability to interact with the cancer target cells in vitro and in vivo, but are easier to manufacture, can be produced at high yields, and are safer by definition. We have designed a multifunctional nanoplatform mimicking exosomes, EMNs, and proved their potential to reach cancer cells with a similar efficient that tumor-derived exosomes but providing important advantages in terms of production methodology and regulations.

Javascript is currently disabled in your browser. Several features of this site will not function whilst javascript is disabled. Received 2 August Published 20 November Volume Pages — Review by Single anonymous peer review.

Matteo, Pavia Italy,. Exosomes are naturally occurring extracellular vesicles released by most mammalian cells in all body fluids. Structurally, exosomes are composed of lipids, proteins, and also several types of RNAs which enable these vesicles to serve as important disease biomarkers. Moreover, exosomes have emerged as novel drug and gene delivery tools owing to their multiple advantages over conventional delivery systems. Recently, increasing attention has been focused on exosomes for the delivery of drugs, including therapeutic recombinant proteins, to various target tissues. Exosomes are also promising vehicles for the delivery of microRNAs and small interfering RNAs, which is usually hampered by rapid degradation of these RNAs, as well as inefficient tissue specificity of currently available delivery strategies.


Request PDF | Exosome-mediated delivery of siRNA in vitro and in vivo | The use RNA extraction was nuclease-free in order to prevent RNase contamination.


Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells

In the s an unexpected gene-silencing phenomena in plants, the later called RNA interference RNAi , perplexed scientists. Following the proof of activity in mammalian cells, small interfering RNAs siRNAs have quickly crept into biomedical research as a new powerful tool for the potential treatment of different human diseases based on altered gene expression. In the past decades, several promising data from ongoing clinical trials have been reported.

Exosome-mimetic nanoplatforms for targeted cancer drug delivery

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siRNA conjugation technology

И все же Сьюзан понимала, что остановить Хейла могут только его представления о чести и честности. Она вспомнила об алгоритме Попрыгунчик. Один раз Грег Хейл уже разрушил планы АНБ. Что мешает ему сделать это еще. Но Танкадо… - размышляла.  - С какой стати такой параноик, как Танкадо, доверился столь ненадежному типу, как Хейл. Сьюзан понимала, что теперь это не имеет никакого значения.

 Странно. Я вчера говорил с. Велел ему сегодня не приходить. Он ничего не сказал о том, что поменялся с тобой дежурством. У Чатрукьяна ком застрял в горле.

Exosome-mediated delivery of sirna in vitro and in vivo pdf free

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Request PDF | Exosome-mediated delivery of siRNA in vitro and in vivo | The use of small interfering RNAs (siRNAs) to induce gene silencing.

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